Reverse Transcriptase & qPCR


Qiagen's Rotor Gene a working horse for qPCR.
Qiagen's Rotor Gene a working horse for qPCR.

  To determine the expression level of certain RNA molecules we use reverse transcription (RT) of RNA preparations followed by quantitative polymerase chain reaction (qPCR). We use these methods as standard technique to determine the endogenous output of transcription factors or their modifying interaction partners like activators or repressors. Our sample material is RNA preparations from human tissue cultured cell lines of different kinds depending on the model system we use.