Initial Screening
Setting up initial screening plates requires protein sample of highest achievable concentration AND protein buffer that can be used for dilution.
Two random screens will be set up using the highest and two 1+1 dilutions. Plates will be checked within the first 1-3 days for their precipitated-to-clear drop ratio.
If the ratio deviates significantly the concentration range will be adjusted. If it seems ok, more initial screens can be set up.
Find a detailed protocol for this test here. Thanks Sandra for putting it together.
Core Screens
find general information here
Composition Tables:
Qiagen JCSG I
Qiagen JCSG II
Qiagen JCSG III
Qiagen JCSG IV
Qiagen JCSG +
Qiagen Cryos
Qiagen Protein Complex
Qiagen NucleiX
KipEGS I
KipEGS II
MD Morpheus
Rigaku Wizard classic 1&2
Rigaku Wizard cryo 1&2
Mosquito short-cut manual
coming soon